The scientific program for Microscopy
and Microanalysis 2003 will
consist of several parts as listed
below. These include: The Premeeting
Symposium on Biophotonics,
Sunday Short Courses
(including some bilingual workshops
in Spanish and English), Special
Events, Symposia, Contributed
Sessions, and Tutorials and Ask The
Experts Sessions. The Symposia will
consist of a mixture of invited and
contributed talks plus a poster
session. Contributed sessions will
also consist of mixtures of talks and posters as warranted by the submitted papers.
When submitting your extended
abstract to Microscopy and
Microanalysis 2003 (see page 19 for
detailed instructions), authors of
invited talks and tutorials should
select only the number
corresponding to that session.
Authors of contributed papers should
select both a first and second choice
(from either the symposia or
contributed sessions). The Program
Committee will use this information
to arrange presentations into
coherent sessions. Inclusion into a
specific session cannot be
guaranteed, but every effort will be made to place your contribution into the most appropriate session.
Throughout the Program, sessions
related to the focus areas
emphasized in this year's program,
Nanotechnology and Biological Applications
of Optical Microscopy.
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Biological Sciences
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1. Laser Microscopy, Fluorescence, Cytomics and Flow Cytometry
Organizers: J. Paul Robinson
and Randall Smith
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This session will focus on correlative
studies on laser microscopies, fluorescence,
flow cytometry, cytomics,
confocal and evanescent wave microscopy
for quantitation of multicomponent
systems. Comparative
methods that involve advances in
fluorescent signals, fluorescent proteins, FRET and other evolving methods are appropriate to this session.
Analysis of light absorption, fluorescence
emission, or compensation of
overlapping signal emission are
appropriate topics for presention in this symposium.
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2. Biochip Microarrays
Organizer: Mark Sanders
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This timely session offers views of
the science and technology of DNA
microarrays and protein arrays
providing up-to-date insights into
microarray research by a distinguished
group of contributors from
many of the hottest academic and
industrial laboratories in this relatively
new field. This session will
focus on sample prep, data/imaging
acquisition, analysis and emerging
technologies in the field. It is intended
for any scientist or biotechnology
professional that wishes to
learn more about the science of
microarrays, how the instrumentation
and technologies are interrelated
and how these technologies
will transform many areas of biomedical and agricultural research in the very near future.
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3. High Resolution Cryo SEM in the Biological Sciences
Organizers: Robert Arpkarian
and Stan Erlandsen
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High Resolution topographic
contrasts of biologically significant
macromolecules and assemblies are
produced by in-lens type FESEMs
fitted with a TEM type cryostage. 1-
10 nm molecular features can be
accurately viewed only when the
sample is immobilized in a vitreous
system and spurious secondary
electrons are kept from the abovelens
detector, as is the case with the
in-lens cryo-FESEMs. This
symposium presents the efforts of a
small number of biomolecular
research labs using in-lens type
FESEMs. In order to share
information and in the hopes of
spurring greater usage of this
ultrahigh resolution technology,
biological chemists will present
images of isolated molecules and
structures of molecular dimension
within the context of complex
biological organization.
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4. Biomaterials
Organizers: Steven Eppell
and Jose Reyes-Gasga
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Biomaterials is now a well know
interdisciplinary field of Materials
Science. It combines metals, polymers,
ceramics, among others, and
science branches such as physics,
biology, medicine and dentistry.
Therefore, the objective of this
symposium is to present the last
results obtained in biomaterials,
enhancing the role played by
electron microscopy in its developing.
Presentations on Orthopedics
(bone, joints, implants), teeth,
foams, ceramics, polymers, materials
composites, computer calculations,
etc, all of them related with
biomaterials and where electron
microscopy have played an important role, are welcome!
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5. Cell Pathology
Organizers: Jay Jerome and
Bill Gunning
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Alterations in cell function are at the
core of pathologic processes. These
alterations include such diverse
things as impairment of DNA repair,
disregulation of signaling pathways,
imbalances in control mechanisms,
mutations in critical enzymes, and
failure of normal responses to stimuli.
This symposium will highlight the
role of microscopy and other imaging
modalities in identifying and
understanding cellular dysfunction
as a contributor to disease. By
necessity, some of these investigations
will also highlight normal
cell processes such as angiogenesis,
apoptosis, and secretion as they
relate to control or exacerbation of
disease states. Contribution of
papers that investigate any aspect of
cell function relative to pathology
are encouraged to submit to this
session.
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6. Biomineralization: Structural Biology, Evolution And Biomimetics
Organizers: Marcos Farina
and Peter Buseck
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Biomineralization is a multidisciplinary
area covering all phenomena
involved in mineral formation by
organisms. More than 60 different
mineral types together with many
structural proteins and polysaccharides,
and specific glycoproteins
have been described and associated
to the biomineralization processes.
Knowledge of structures of biominerals
and biomineralization processes
helped in the design of specific
molecular probes (e.g. magnetoferritin), as well as bone substitutes (e.g. several bioceramics composites). The more we correlate structural biology with the mineral counterpart in biomineralized tissues, the more we contribute to the understanding of possible evolutionary pathways in biomineralization and to the development of new materials for applications following a biomimetical approach.
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7. Biological Microanalysis
Organizers: Ann LeFurgey
and Peter Ingram
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State-of-the-art analytical imaging
methods will be discussed together
with their strengths and limitations
in basic biological, biomedical and
clinical research. Papers addressing
both qualitative and quantitative
precision of spectrum imaging are
especially solicited. Several keynote
speakers will be invited to review
recent advances that explore useful
correlations between such disparate
techniques as X-ray, Fourier Transform
Infrared, Raman, Secondary
Ion Mass Spectrometry, Coherent
Anti-Stokes Raman Scattering
(CARS), Fluorescence Resonance
Energy Transfer, Electron Energy
Loss Spectroscopy etc. as applied to important biomedical issues.
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8. Modern Microscopy in Parasitology
Organizer: Wanderley de
Souza
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The aims of this Symposium are to
show how microscopy has contributed
to the present knowledge of
the structural organization and cell
biology of parasitic protozoa and
nematodes, and to discuss the
potentialities of new techniques to
the study of parasites. The Symposium
will cover results on microscopic
analysis of the strucutral
organization, cell surface, intracellular
trafficking, and vesicle fusion in
parasitic protozoa, as well as the
structure of the cuticle of nematodes.
We will also enphasize the
various methodologies that can be
used to study parasites. Emphasis
will be given to the use of Confocal
Microsocpy, Atomic Force Microcopy, Freeze-Fracture, High Resolution Scanning Microscopy, Immunocytochenistry and In Situ Hybridization.
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Physical Sciences
Symposia
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9. Off-Axis Electron Holography And Related Interferometric Methods
Organizers: Molly McCartney
and Michael Lehmann
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Off-axis electron holography and
related interferometric methods can
overcome experimental factors
limiting high-resolution transmission
electron microscopy. In addition,
the sensitivity of the phase of the
electron wave can be exploited to
yield unique information on electrostatic
and magnetic fields. Previously
only available in highly specialized
laboratories, holographic methods
have recently found a wide range of
applications in physics, material
science, and biology. On the nanometer
scale, holography provides a
means to image and measure magnetic
fields and electrostatic potential,
and exciting in-situ experiments
have been demonstrated. At
atomic resolution, aberration correction in combination with perfect zero-loss filtering can contribute to a fundamental understanding of electron-object interactions. This symposium will provide a forum for a discussion of all new developments and applications.
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10. Electron Microscopy of Nanomaterials: Novel Techniques, Structures and Properties
Organizers: Pulickel M.
Ajayan, Elizabeth Dickey,
Daniel Ugarte, ZL Wang, and
Jim Zuo
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Rapid progress in nano-science and
nano-technology presents new
challenges and new opportunities for
the development of advanced microscopy
techniques. Unique to nanostructures
are the small feature
lengths and large surface/interface
areas. Characterization ranges from
quantifying morphology and size
distributions to measuring local
structure and chemistry at the
atomic scale. This symposium will
provide a forum for discussing complementary
microscopy for characterizing
nanomaterials and nanostructured
devices. Topics will
include quantitative imaging, subnanometer
spectroscopy, 3-D reconstruction,
interface determination
and electron diffraction as applied to nanomaterial analysis.
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11. The Changing Role of Atom Probes: Microscopes for the Nanotechnology Era
Organizers: Tom Kelly and
David J. Larson
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This symposium will highlight the
latest applications of atom probes
and consider the role they may play
in such industries as semiconductor
microelectronics, magnetic storage,
and advanced metals. Atom probes
are gaining wider application and
attention as their unique capabilities become essential for work at the nanoscale. One of the primary reasons for this is the continuing push to make devices smaller and smaller.
"Atomic-scale engineering" is becoming
a reality and practical methods
for monitoring device fabrication
on this scale will be essential. There
will also be a major emphasis on
exploration of new or evolving
applications.
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12. Polymers
Organizers: Caribay Urbina
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Polymer characterization strives to
study relationships between the
structure and properties of these
materials. Electron Microscopy
techniques play an important role in characterization studies. This symposium will cover traditional and novel techniques used to explore the ultrastructure of polymers.
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13. Electron Backscatter Diffraction: New Developments and Applications
Organizers: Joe Michael and
Val Randle
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Electron backscatter diffraction
(EBSD) has developed rapidly into
an important technique for crystallographic
characterization of materials
in the SEM. EBSD is a tool
that has applications in the areas of
orientation studies and phase identification.
This session will focus on
advances in our fundamental understanding
of the origin of EBSD patterns, advances in the technique of
EBSD (hardware and software) and
applications of EBSD to materials of technological or commercial importance.
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14. Semiconductors and Magnetic Materials
Organizers: John Mardinly
and Bob Sinclair
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Semiconductors and Magnetic Materials
are the key to driving future
developments in computational power,
information storage and retrieval,
and communication and streaming
data both through wired and wireless
Internet protocols. Microscopy
and microanalysis are key tools for
understanding the microstructure
that links the processing and performance of devices built from these materials. This symposium will cover applications of every sort of microscopy and microanalysis for understanding the micro characteristics of semiconductors and magnetic materials, as well as devices and systems fabricated from these materials.
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15. Metallography and Physical Metallurgy
Organizers: George
VanderVoort and Juan
Asensio-Lozano
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There are well known relationships
between the composition of metals
and alloys, their processing, the
resulting microstructure, mechanical properties and service performance.
Microstructural analysis is like a
fulcrum between either side of these
relationships. One can simply evaluate
the performance or the properties
of metals and alloys as a function
of composition and processing;
but without understanding the microstructural
aspects, the knowledge
obtained is empirical at best. This
symposium will present leading researchers
discussing how a knowledge
of microstructure, and efforts
to control microstructure, have lead
to a better understanding of fundamental
issues in physical metallurgy
and development of better metals
and alloys.
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16. Scanned Probe Microscopy: Tools for Establishing Structure-Property Relationships
Organizers: Phillip E. Russell
and Louis T. Germinario
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Various forms of Scanned Probe
Microscopy are now providing high
spatial resolution structure-property
relationship data from a very wide
range of materials from disciplines
such as materials science and engineering,
biology, chemistry and
physics. Technique fundamentals
and recent developments, as well as
applications from polymer, biomaterials,
semiconductor and pharmaceutical
industries will be highlighted
in this symposium.
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17. Quantitative X-ray Microanalysis
Organizers: Raynald Gauvin
and Eric Lifshin
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The aim of this symposium is to
present the latest new developments in X-Ray microanalysis in the electron microprobe, SEM and ESEM.
This area continues to grow because
of progress in instrumentation, modelling
techniques and software making
it possible to obtain useful results
from a greater range of samples
examined over a broad range of
experimental conditions. Topics of
particular interest include: software
relating to new correction procedures,
spectrum processing and
Monte Carlo simulations of X-Ray
spectra. X-Ray microanalysis of light
elements and spectra obtained at
low operating voltages including
operation in FE-SEM's. Special
considerations for X-Ray analysis in
the ESEM and VP-SEM, including
spectral artefacts, optimising spatial
resolution, understanding insulating
samples and general procedures for
quantitative analysis. Data from the
X-Ray microanalysis of rough surfaces,
porous materials and multilayer
thin film structures, better
measurement and computation of
fundamental parameters describing
X-Ray emission, and electron diffusion
with the result of improved
accuracy and precision of quantitative
X-Ray microanalysis. In this
symposium, we hope to show that
new X-Ray microanalysis methods
continue to be developed to meet
the characterisation needs of investigators in numerous fields from microelectronics to mineralogy.
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18. Image Analysis and Digital Microscopy for Materials Science
Organizers: Sidnei Paciornik
and Barry Jenkins
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The growth in computer technology,
image processing methodology and
microscope automation has created
a digital microscopy revolution.
Light microscopy has seen great
growth in the application of stereological,
metrological and mathematical
morphological procedures to
the study of materials related problems
concerning microstructural and
fractographic characterization. Microscope
automation and control and
digitization of images has been central
to atom probe microscopy, is
critical for spectral imaging by SEM
or TEM and have been crucial in
extending the resolution limits of
HRTEM. Now, these improvements
are being carried into light microscopy
and light microscopy stereological
and mathematical morphological
developments are entering
the domain of SEM, TEM and atom
probe techniques. This session
invites speakers to present examples
of the integration of these new
technologies into materials science
applications, with emphasis on new ideas, new capabilities, techniques, and analytical solutions.
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19. Microscopy in Archeology and Art
Organizers: Victor M.
Castano, Russel Chianelli, and
Ventura Rodriguez
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This symposium is aimed to materials
scientists, optical and electron
microscopists, archaeologists, technicians,
historians and all the professionals
whose work is somehow
related to the identification, dating,
classification, analysis and preservation
of archaeological materials
and pieces of art, both ancient and
modern. Contributions are expected
in, but not restricted to, the following
topics: microscopy analysis of
ancient metallurgy objects, composition
of prehispanic murals, idenification of prehistoric remains,
analysis of painting techniques by
microscopy methods, high resolution
microscopy of nanophases in ancient
materials, etc. This symposium will
include 4 invited lectures by worldrecognized
experts, as well as contributed
presentations and a poster
session.
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20. Electron Microscopy on Phase Transformations and Microstructures in Alloys
Organizers: Francisco C.
Lovey and David Rios Jara
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This symposium will bring together
people working in phase transformation,
microstructures, and defects
in alloys. This involves characterization
of stable and metastable
phases, interfaces, precipitates, and
defects by electron diffraction, diffraction
contrast, CBED, EELS, EDS
and HREM. The relation of the microstructures
with mechanical properties
will also be of interest for this
symposium.
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21. Characterization of Ultra-Fine and Nanoparticulates
Organizers: Lawrence E. Murr
and John J. Bang
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There is world wide interest currently
in the development and utilization
of ultra-fine and nanoparticulate
materials in special processes to
promote unique properties and
performance. It is often very difficult
to collect these particulates or to
characterize them in a product
environment. This symposium seeks to focus on methods of characterization for extremely small particulates.
Special interest in the collection
of particulates in the atmosphere
and their characterization will
also be included.
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22. Welding Metallography
Organizers: John C. McClure
and Lawrence E. Murr
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This symposium seeks to bring together
those interested in exploring
the microstructures associated with
a variety of welding processes, the
control of weld microstructures in
process optimization, and a comparison
of microstructural issues. New
welding
processes, such as friction
welding and especially friction-stir
welding, will be a particular focus.
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23. Standards in Metallography, Microanalysis and Microscopy
Organizers: John J. Friel and
Robert C. Nester
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Obtaining precise, reproducible data
requires development of standard
practices and procedures. This covers
the obvious need for calibration
of instruments, proper sampling and
data analysis, but also extends to
development of preparation methods
that yield the true structure, development
of analytical methods, development
of standard materials and
a host of other issues. This symposium
seeks to bring together those
interested in discussing issues related
to the application and generation
of precise, unbiased, reproducible
test results using light microscopy,
image analysis, electronic imaging, electron microscopy, hardness and other commonly encountered equipment in the materials research or production test laboratory.
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24. Metallography: Preparation and Application
Organizers: William F. Gale
and Allan J. Lockley
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Metallography is fundamental to the study of metals and materials.
Development of good preparation
methods and good ways to reveal
the structure by etching, either in
black and white or color, or using
optical methods, e.g., polarized
light, dark field or differential interference
contrast, is an ongoing task,
especially for newly developed
materials. This session seeks to
bring together those interested in
sharing experiences related to the
development and use of specimen
preparation methods for all types of metals, ceramics or polymeric materials.
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Advances in Instrumentation and Techniques Symposia
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25. Advances in Scanning Probe Microscopy and Microanalysis
Organizer: Rodrigo Prioli
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This symposium will cover new
achievements in application, theory
and instrumentation of all scanning
probe microscope techniques. In the
meeting, discoveries in the scanning
tunneling, atomic force, friction,
magnetic, chemical, thermal, and
near field optical microscopy are
welcome. Applications of the above
mentioned techniques should give
emphasis not only on the study of
physical and chemical properties of
solid surfaces but also on the imaging of biological systems.
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26. Advances in Quantitative Optical Microscopy, a symposium in honor of Watt Webb
Organizers: David Gross and
Warren Zipfel
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This session will honor Professor
Watt W. Webb's longstanding contributions
to quantitative optical microscopy,
biological fluorescence
spectroscopy and biophysics. It will
highlight areas in which the Webb
laboratory has specialized over the
past three decades, with topics
ranging from macromolecular mobility
and cell signaling to techniques
pioneered in the laboratory such as
fluorescence correlation spectroscopy and multiphoton microscopy.
Talks will focus on applications of
optical techniques to living cell
dynamics covering both the biological questions at hand and the instrumentation designed to answer them.
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27. Electron Tomography: Recent Advances and Applications
Organizers: Mike Marko and Daniela Nicastro
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Electron tomography is rapidly gaining
in popularity in both biology and
materials science. This is due in
large part to increased ease of data
collection from improved microscope
automation software and specimen
stages. As the ideal method for 3-D
ultrastructural study of single
examples of asymetric objects in the
size range of about 100 to 1000
nanometers, tomography has wide
application. Technical developments
include cryo-tomography, rapid data
collection, and application of imaging
modes such as high-angle annular
dark field STEM and energy
filtering. Advances are also being
made in alignment and reconstruction
algorithms, as well as in improved
interpretation and analysis of
tomograms. Invited speakers will
represent current leaders in technical development, as well as investigators with exciting applications.
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28. Advances in Correlative Bio-Imaging Techniques
Organizer: Bob Price
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In today's research environment
questions in the biological sciences
are frequently addressed by multiple
techniques that include the use of
traditional microscopy instrumentation
in combination with biochemical
and molecular techniques. Even
though the emphasis of this session
will focus on the use of correlative
microscopy techniques such as light,
confocal and electron microscopy,
the overall theme of the presentations
will also address how these
microscopy techniques are being
used with other bio-imaging techniques
such as micro-CTs, DNA
arrays and real-time RT-PCR, to
answer complex questions in a number
of biological systems. Meeting
attendees are urged to contribute
both platform and poster presentations to supplement the invited talks in the session.
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29. Advances in Preparative Technique for Bio-Imaging via LM, SEM, and TEM
Organizer: Ralph Albrecht
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Research protocols that utilize microscopy
to provide answers to biological
questions often require information
from several microscopic
modes. Current advances in preparative
methodology for biological
studies employ technology that
permits the sequential examination
of specimens in several microscopic
modes. Specimens stained and/or
labeled for examination while living,
must be prepared for subsequent
examination at higher levels of spatial
resolution by SEM or TEM. This
requires labeling technology that is
compatible with, and provides
information from, more than one
microscopic mode and generally at
different levels of spatial resolution.
It also requires technology that
maintains molecular and sub-molecular
structure often in the face of
imaging at both low and high accelerating
voltages. Advances in chemical
and cryo fixation, direct observation
of hydrated specimens, coating
technology, and the development of
staining and labeling procedures for
correlative LM, TEM, and SEM will be
addressed. Contributed platform
presentations and posters dealing
with novel preparative approaches
are encouraged.
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30. Microscopy, Microanalysis and Image Analysis in the Pharmaceutical Industry
Organizers: Barbara Hartman
and Jeff Horn
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Pharmaceutical research and development
laboratories are at the
forefront of science. Much of the
work performed in pharmaceutical
microscopy and microanalytical labs
is similar to that done elsewhere,
however, there are specialized technologies
and themes that are of
particular value to microscopists in
the industry. It is the objective of
this symposium to present a variety
of biological and materials science
applications of significance to the
pharmaceutical community. Invited
speakers will include current leaders
in their respective disciplines. Additionally,
as in previous meetings, an
informal forum will be provided for
sharing of thoughts and strategies
related to regulatory and other
issues faced in our laboratories.
Contributed papers for platform or
poster presentation on related topics are encouraged and welcome.
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31. Advances in New Detectors for X-ray Spectrometry
Organizers: John Henry Scott
and Dale Newbury
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Continuing progress in high resolution
microcalorimetry and high
count rate silicon drift detectors for
x-ray spectrometry will be considered.
Papers that describe experience
gained with these spectrometers
installed in working laboratories
are especially welcome.
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32. Spectral Imaging: Analysis, Visualization, and Applications
Organizers: Ian Anderson and
Paul Kotula
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Spectral images provide chemical
information that is spatially resolved from a 2D region of a microstructure.
Spectral imaging (EDXS and
EELS) systems have been commercially available with SEMs and STEMs and are finding their way into more laboratories around the world.
The challenge with spectral image
data sets, which typically contain in
excess of tens of millions of data
points, is to both effectively acquire
the data and then extract the useful
chemical information with minimal
effort. In this symposium we seek
papers dealing with spectral image
acquisition, analysis, and more
general application issues. This
includes conventional analyses such
as mapping and other novel visualization
approaches as well as statistical
analysis methods. Additionally,
we seek examples that illustrate
both success stories as well as pit
falls of the application of spectral
imaging to microanalysis problems. This symposium will follow on from the MAS Special Topics Workshop on spectral imaging and data analysis in April 2003 at NIST and the results of the discussions from that workshop will be presented.
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33. High Spatial and Energy Resolution Electron Energy Loss Spectroscopy
Organizers: Nigel Browning
and Gerd Duscher
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This symposium will focus on the
application of high spatial resolution
and/or high energy resolution electron
energy loss spectroscopy to
both materials and biological
sciences. Topics that will be covered
will include: the development of new
instrumentation (e.g. monochromators
and aberration correctors),
theoretical methods for interpreting
fine-structures, quantitative comp-
ositional analysis, comparison with
other spectroscopic methods and
correlation with high resolution
imaging. Applications of EELS that focus on the link between electronic structures and properties are encouraged.
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34. Electron Microscopy of Dynamic Processes
Organizers: Frances M Ross
and Mark Yeadon
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Electron microscopy, with its
combination of high spatial and
temporal resolution, is a unique tool with which to study surface and bulk processes such as deposition, phase transformations and chemical reactions.
The field of in situ microscopy
is advancing rapidly at present due
to recent developments allowing
TEM in liquid environments, new
specimen designs incorporating
MEMS technology, and a greater
emphasis on extracting quantitative measurements from in situ observations.
Furthermore, the promise of
aberration correction in allowing a
larger polepiece gap is encouraging
the design of more complex future
experiments for the TEM. This symposium
solicits applications of in situ
TEM, LEEM, STEM, ESEM and optical
microscopy, including analytical and
diffraction studies, to the
understanding of important dynamic
processes, as well as developments
in the areas of microscope, specimen
and holder design and real
time data analysis. We anticipate a
valuable exchange of scientific
results and innovative experimental designs which will set the direction for future in situ studies.
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35. Focus on Infrared and Raman Microanalysis
Organizers: Edgar Etz and
Derek J. Gardiner
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This Symposium will focus on the
current state of vibrational microanalysis
in the materials, biological,
and analytical sciences. Topics will
include: Fundamentals of inelastic
scattering, advances in instrumentation for IR and Raman microspectroscopy/ microscopy, with emphasis on synchrotron microIR and nearfield IR and Raman microscopy.
Highlighted will be the latest in
chemical state spectroscopic
imaging, including instrument performance, probes for biological/ medical applications, and the analysis of complex data sets. Characterization of advanced materials, in the fields of semiconductors, ceramics, polymers and composites.
Focus on applications to tissue analysis
at the cellular level, to environmental
studies, the geological sciences,
forensics, and art and archaeology.
Quantitation of spectra and
compositional maps/images from IR
and Raman probing, including instrument
calibration and standardization
of output data. Papers on
related topics (e.g., photoluminescence and fluorescence microspectroscopy and imaging) will also be considered.
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36. Focused Ion Beam Instrumentation and Techniques for Biological and Materials Sciences
Organizers: Joe Michael and
Hendrik O. Colijn
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Focused ion beam instrumentation is
now more common in the microscopy
laboratory. The unique imaging
and milling capability of the FIB
has made it an ideal tool for sitespecific
preparation of samples for
SEM or TEM. Also, the ability to
introduce specific gasses for deposition
of various materials and sample
modification has provided new and
interesting applications. This symposium
encourages abstracts that
discuss the development of new FIB
instrumentation and techniques for
materials and biological sciences.
Papers are also encouraged in the
area of fabrication of three-dimensional micro or nanostructures through additive processing with FIB instrumentation.
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37. Advances in High-Resolution Imaging
Organizers: Larry Allard and
Michael O'Keefe
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High-resolution electron microscopy
(both TEM and STEM) has recently
achieved sub-Angstrom resolution.
Methods used to enhance resolution
include hardware correction (CS
correctors applied to the probeforming
STEM lens or TEM objective
lens), software correction (focal
series reconstruction) and approaches
such as holography that combine
specialized hardware with reconstruction
software. This session
will cover recent advances and applications
of such techniques to practical
imaging, as well as techniques
for improving the TEM information limit (monochromators, CC correctors).
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38. Low Voltage Microscopy and Microanalysis
Organizers: David Joy and
Raynald Gauvin
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This symposium will consider the
expanding frontiers of low voltage
electron microscopy. Topics to be
highlighted will include, transmission
and scanning transmission microscopy
at low voltage; ultra-low voltage
imaging; novel isntrumentation
for low voltage operation; theories
of electron scattering, stopping
power, and contrast formation at low and ultra-low voltages.
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39. Industrial Applications of Microscopy - Techniques for the Real World
Organizers: Zhigang Li and
Janet Woodward
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Many microscopy techniques have
become mature and have been
broadly used throughout industry.
Microscopists from the diverse industrial sectors are invited to share their years of successful experiences in using various microscopy techniques.
Light, scanning and transmission
electron microscopy and
associated techniques will be covered
in this symposium to illustrate
how these techniques have been
used in solving real world problems. Automation, quantitative measurements, 3D imaging, combination of different microscopy techniques will be discussed. Another major focus of the symposium will be the prospect of the broader applications of microscopy in industry and the challenges we will face in twenty-first century.
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40. Innovative Sample Preparation for TEM and HRSEM for the Physical Sciences: A Symposium in Honor of Reza Alani
Organizers: Scott D. Walck
and Shane Roberts
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This symposium focuses on creative
and innovative sample preparation
techniques, problems and their solutions
for TEM and HRSEM. Topics of
interest include, but are not limited
to, preparation of cross section and
plan view samples, sample cleaning,
sample preservation, control of artifacts
and damage, specialized specimen
handling and exchange, in-situ
processing, ion milling, dimpling,
preparation of thin film analytical
standards, and particular preparation techniques for specialized imaging or micro-analytical techniques.
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41. Teaching Microscopy and Imaging in the Digital Age
Organizer: Steve Barlow
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Computer technology is changing
the ways we access equipment, view
samples, and record or manage
images. Computer-controlled microscopes and digital imaging have created many new training methods.
In addition, these changes make it
possible for classroom students to
analyze microscopy data and images
without requiring access to expensive
microscopes. This session will
look at new training approaches,
such as virtual microscopes and
telemicroscopy, and new teaching
tools, such as CDs, DVDs, and
computer software. The session will discuss using digital technology to create laboratory training protocols and to teach classroom analysis of data contained in digital images.
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Technologists' Forum
Organizer: Cathy Johnson
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42. Platform Session: "Diverse
Techniques for Complete
Specimen Analysis &
Characterization"
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This symposium will focus on the use
and integration of multiple analytical
techniques to fully characterize a
specimen. Emphasis will be placed
on selection of the appropriate
method(s) to obtain the information
required, and maximizing data output
in order to address the primary
concern(s) of interest. Invited
papers will present both research
and industrial scenarios, and will
include materials as well as biological samples. Contributions for poster presentations are welcome.
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43. Special Topics: "Immunology
201: Advanced Techniques"
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This session will feature invited
speakers who will cover two techniques
in depth: antigen retrieval and
FISH. Antigen retrieval is an important
addition to our tools for immunolocalization
studies by providing
ways to unmask reactive epitopes
for increased primary antibody
binding to the tissue antigen. There
are numerous published methods for
antigen retrieval; however, no one
method works for all proteins. The
methodology must be approached
carefully, since false positive labeling and destruction of antigenic epitopes are both possible. The use of appropriate controls is mandatory when using antigen retrieval in immunolabeling.
Fluorescence in situ
hybridization (FISH) is a tool to
detect chromosomal abnormalities in
humans or determine the genotype
of a knockout or transgenic animal.
Fluorescently labeled sequences of
single-stranded DNA are bound
(hybridized) to the complementary
DNA sequences of interest, thus
allowing identification of chromosomes
or genes based on color,
rather than banding patterns as in
traditional karyotyping. FISH can be
performed on either dividing or
nondividing cells, making it more
adaptable than other techniques
used to study chromosomes.
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44. Roundtable Discussion:
"Computer Security in
Today's Microscopy Facility"
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Security of individual computers,
including control of access, is a
major concern for microscopy
laboratory managers. Panel
members will discuss protection
from computer viruses, as well as
management of user logins and
remote logins to instruments and file servers. Interactive discussion with the audience will follow.
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Topics for Contributed
Sessions
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Biological Sciences
45. Biological Microanalysis
46. Biological Ultrastructure (Cells, Tissues, Organ Systems)
47. Biomedical Applications
48. Biopolymers and Biomemetics
49. Blood/Immunology
50. Botany
51. Correlative Microscopy
52. Developmental/Reproductive Biology
53. Entomology
54. Microbiology
55. Neurobiology
56. Parasitology
57. Pathology
Physical Sciences
58. Advanced Composites
59. Films/Coatings
60. Geology/Mineralogy
61. Ferroelectrics
62. Modulated Structures
63. Oxidation/ Corrosion
64. Phase Transformation in Metals, Alloys and Ceramics
65. Radiation Effects in Materials
66. Semiconductors
67. Surfaces/Interfaces
Advances in Instrumentationand Techniques
68. Auger Electron Microscopy
69. Compositional Mapping
70. Computational Methods for Microscopy and Microanalysis
71. Cytochemistry (Light and Electron Histochemistry, Immunohistochemistry, In-Stu Hybridization)
72. Diffraction Techniques
73. Electron Crystallography
74. Electron Holography
75. Field Ion Microscopy
76. High Resolution Electron Microscopy
77. Image Simulation and Image Processing Techniques
78. In Situ Microscopy Techniques
79. Instrument Performance
80. Live Cell Imaging
81. Microbeam Mass Spectroscopy
82. Molecular Spectroscopy
83. Secondary Ion Mass Spectroscopy
84. SEM
85. Stereology
86. XRF/XRD Techniques
87. X-ray and Optical Crystallography
88. Bright Field and Fluorescence Microscopy
89. Corporate Session
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