SIS block copolymer

by R.J. Spontack and R. Thomann

Meeting Symposia

Categories for Scientific Papers and Symposia

Documented below are the Planned Symposia for the 2004 meeting.

The scientific program for Microscopy and Microanalysis 2004 will consist of several parts as listed below. These include: The Premeeting Symposium, Sunday Short Courses , Special Events, Symposia, Contributed Sessions, and Tutorials. The Symposia will consist of a mixture of invited and contributed talks plus a poster session. Contributed sessions will also consist of mixtures of talks and posters as warranted by the submitted papers.

When submitting your extended abstract to Microscopy and Microanalysis 2004, authors of invited talks and tutorials should select only the number corresponding to that session. Authors of contributed papers should select both a first and second choice (from either the symposia or contributed sessions). The Program Committee will use this information to arrange presentations into coherent sessions. Inclusion into a specific session cannot be guaranteed, but every effort will be made to place your contribution into the most appropriate session.

The deadline for receipt of all papers is February 16, 2004.


Biological Sciences
B01 Imaging Technology in the Study of Cardiovascular Development and Disease
Organizers: Rob Gourdie, Bob Price
Featured Invited Speakers: Mark Sussman, Kersti Linask
Cardiovascular disease is a primary factor in premature death in most developed countries and in the US alone more than 61 million people suffer from some form of heart disease including congenital malformations, stroke, high blood pressure, congestive heart failure and atherosclerosis. Statistics released by the American Heart Association indicate that each year in the United States these diseases result in 39% of the total deaths and cost more than 352 billion dollars in medical costs and disabilities. This symposium will look at several studies that have used imaging technology to study cardiovascular development and disease and will include several invited presentations from noted researchers in the field that will subsequently be published in an issue of Microscopy and Microanalysis. Contributed papers for both platform and poster presentations are also welcome including those that would normally be submitted to the Vascular Casting Focused Interest Group Session.
B02 Biomaterials
Organizers: Steven Eppell, Eric Henderson
Featured Invited Speakers: Larry Bottomley, Vince Moy
Biomaterials research is an interdisciplinary field requiring expertise in both physical and biological sciences. Successful biomaterials provide the necessary mechanical and chemical properties for medical and drug delivery devices while eliciting appropriate biological host responses. Additionally, biomaterials represent a vast conceptual landscape for developments in bionanotechnology. It is abundantly clear that the natural materials we seek to emulate, replace or integrate into composite systems have rich structural geometries emerging from nanoscale patterns and principles and blossoming into complex macroscale assemblages. Nature provides a multitude of design motifs for both passive and active nanoscale materials such as bone, retina, synaptic membranes and actin/myosin muscle fibrils. The biomimetic approach has driven investigators to use TEM, scanned local probes, and diffraction techniques to ascertain the salient details of these natural materials and to probe for success or failure in creating synthetic substitutes. This year's symposium will focus on these nanoscale biomimetic studies. While all biomaterials oriented studies are welcome, our emphasis will be on the problems and benefits gleaned from looking at natural and synthetic systems at the nanoscale. Here, nanoscale is defined by features of interest with at least one dimension between 1-1000 nm.
B03 Microscopy of Plant Pathogenic Microbes and Their Interactions with Host Plants
Organizers: Charles W. Mims, Kirk Czymmek
Featured Invited Speakers: Richard J. Howard, Elliot Kitajima
This symposium focuses upon the contributions of various forms of microscopy to the study of plant pathogenic microbes and their interactions with host plants. Contributions relating to the study of plant pathogenic fungi, viruses, bacteria and nematodes and their interactions with host plants are welcomed. Invited speakers include internationally known experts in their respective fields of study. Examples of topics to be discussed include modern microscopic methods for imaging fungal pathogens and host-pathogen interactions, ultrastructure of fungal haustoria, TEM of plant viruses and TEM and SEM of Xylella fastidiosa in the xylem of various host plants.
B04 Microscopic Analysis of Nervous System Development and Function
Organizer: Deanna Smith
Featured Invited Speakers:Li-Huei Tsai, Richard Vallee
The polarized spatial organization of the neuron, with its long and often elaborate axonal and dendritic projections, provides unique opportunities to study mechanisms regulating cell compartmentalization and morphogenesis. At the same time, their morphology is an impediment to biochemical analysis. Because of this, researchers have often turned to microscopy to explore neuronal development and differentiation. The functional organization of the mature synapse has also been probed extensively using this approach. This symposium will solicit papers related to the use of microscopic analysis to elucidate molecular mechanisms regulating neuronal migration, neurite formation, synaptogenesis, and synaptic function. Immunofluorescence methods, time-lapse imaging of living cells, and analysis of calcium signaling are examples of topics to be discussed.
B05 3D Electron Microscopy of Macromolecules: Unveiling Structural/Functional Relationships through Imaging Conformational Changes
Organizers: Gina Sosinsky, Teresa Ruiz
Featured Invited Speakers: Christopher W. Akey, Michael Radermacher
One of the ultimate goals in biology is to achieve an understanding of the structure/function relationships that govern the organization of macromolecular complexes. Technical advances have turned three-dimensional electron microscopy into an ideal tool to elucidate structural mechanisms by examining tertiary and secondary structure. Using either cryoelectron microscopy, alternative staining or fast freezing techniques, it is possible to trap macromolecules in distinct states that can be directly correlated as functional intermediates. This symposium will highlight recent developments and structures illustrating a range of macromolecular complexes that span the extent of cell biology.
B06 Microscopy in Microbiology
Organizers: Robert Simmons, Cynthia Goldsmith
Featured Invited Speakers: Tom Geisbert, Sara Miller
Microscopy has long been a vital component of microbiological research. High-resolution techniques using TEM, SEM and AFM are often employed to examine the physical structure as well as molecular mechanisms of individual organisms, including viruses, bacteria, fungi and parasites. Light, confocal and deconvolution microscopy along with SEM and TEM are powerful tools for studies of the relationships of microorganisms with one another and with their environments. Interactions between microorganisms and hosts play a major role in the infectious disease process and may result in granulomatous inflammation, intracellular inclusion bodies or autoimmune phenomena. Microbial communities also may form components of many food production and industrial processes as well as waste remediation systems. This symposium is planned to demonstrate the use of microscopy to explore a variety of questions about microorganisms and their interactions with the world around them.
B07 Advances in Imaging of Cytoskeletal Dynamics, Structure, Regulation, and Functions
Organizer: Heide Schatten
Featured Invited Speakers: William M. Bement, Lester I. Binder, Tobias I. Baskin
The cytoskeleton plays important roles in a variety of different cell system and it is critical for cell maintenance and metabolic activities. Microfilaments, intermediate filaments, microtubules, their organizing centers, and numerous associated proteins as well as regulatory enzymes carry out critical and varied cytoskeletal functions such as signal transduction, transport of cell organelles and molecular cell components, secretion, cell motility, muscle contraction, and growth of plant cell walls. Cytoskeletal abnormalities and dysfunctions of cytoskeletal regulation are contributing causes for many diseases including cancer and Alzheimer's disease. Cytoskeletal abnormalities have also been implicated in infertility and developmental defects. This symposium will highlight the various imaging methods that are being used on live and fixed cells to study cytoskeletal dynamics, structure, regulation, and functions as well as abnormalities in a great variety of cells including neuronal cells, microorganisms, embryonic cells, plant cells, and other cell systems. Exciting new developments in techniques, molecular imaging methods, and instrumentation including multiphoton microscopy, field emission scanning electron microscopy, atomic force microscopy, and video microscopy combined with immunological probes have allowed detailed insights into cytoskeletal dynamics and molecular interactions of cytoskeletal components and cell organelles leading to significant contributions and advancing several fields of science.
Physical Sciences Symposia
P01 Interfaces: A Symposium in Honor of Manfred Ruhle
Organizers: Frank Ernst, John Bruley
Featured Invited Speakers: W. Mader, Y. Ikuhara
The properties of many materials, particularly when engineered on the nanometer scale, critically depend on the properties of grain boundaries and heterointerfaces (phase boundaries). Recent developments in instrumentation and methods of TEM (transmission electron microscopy) have provided powerful tools for assessing the atomistic structure, electronic structure, and spatial distribution of atomic species at such "internal interfaces" in solid materials. For example, techniques of quantitative high-resolution TEM, analysis of near-edge structure in electron energy-loss spectra, or techniques of energy-filtering TEM provide very detailed and complementary information on interfaces and enable sensitive comparisons with the results of atomistic simulations. significant contributions to developing advanced quantitative TEM techniques for analyzing interfaces have been made by Manfred Ruehle and his group at the Max-Planck-Institut fuer Metallforschung in Stuttgart, Germany. This symposium is dedicated to applications of these techniques to internal interfaces in all classes of materials and further developments of quantitative TEM techniques that provide fundamental insight into the atomistic structure, composition, and bonding of grain boundaries and heterointerfaces. The emphasis of this symposium will be the correlation between interface structure and properties.
P02 Characterization of Novel Nanostructures for Applications in Sensing, Nanoelectronics and Biotechnology
Organizers: Perena Gouma, Zhong Lin Wang
Featured Invited Speakers: P.M. Ajayan, V.P. Dravid, Jun Liu
There is a wealth of novel nanostructured materials, including nanoparticles, nanowires, nanoshells, nanotubes and nanobelts, that offer high surface areas and unique physical-chemical properties that make them suitable for sensing and NEMS applications. Metals and alloys, semiconductors and semiconducting oxides, as well as composite nanostructures are currently being used to build the latest generation of separation membranes, electrodes, chemical detectors, biosensing probes, and bio-imaging tools. Electron microscopy is an essential tool for characterizing these novel materials. This symposium will solicit papers in the broader areas of nanomaterials characterization, phase transformations and interface phenomena induced during sensing, including: (i) in-situ processing, imaging, and analysis of nanomaterials in an environmental cell; (ii) gas-nanomaterial interactions; (iii) interaction of inorganic, organic, and biological materials in composite nanostructures; and (iv) polymorphism and structural stability of nanomaterials and nanostructures. Invited speakers include internationally recognized experts in nanomaterials characterization with a focus on functional materials, from academia, industry and research laboratories.
P03 Nanostructure and Dynamics of Molecular Assemblies: Biomembranes, Proteins, Surfactants, Polymers and Liquidcrystals
Organizers: Dganit Danino, Joe Zasadzinski, Dave Siegel
Featured Invited Speakers: Alisdair C. Steven, Katarina Edwards
This symposium will highlight the use of electron microscopy (mainly cryo-TEM) to study dynamic processes and mechanisms in self-assembling systems. Self-assembly is a general, fundamental process whereby physical interactions control the spontaneous association of molecules into organized, nano-scale, dynamic structures. Self-assembly governs the formation of biological membranes and biological fluids like bile and lung surfactant, as well as the structure of complex fluids such as micelles, liquid crystals, multiblock copolymers, and mixtures of these materials. These materials find numerous applications in industry (e.g., detergents, drug delivery vehicles, personal care products, electronics), and construction of new nanostructured materials often exploits self-assembly as a means of obtaining desirable properties, such as shape, ordering, size distribution, viscosity, and surface tension. Electron microscopy is an appropriate tool for studying the structure of these materials, because of the nanometer scale in at least one dimension and the need to understand the structures in terms of the interactions of their constituent molecules. In this symposium, we wish to emphasize that electron microscopy is also an appropriate tool for understanding dynamic processes (e.g., the mechanisms of structural transformations and phase transitions), especially when non-perturbative specimen preparation methods are used (as in cryo-TEM and freezefracture electron microscopy).
P04 Microscopy and Microanalysis in Catalysis
Organizers: Pratibha L. Gai, Steven Bradley
Featured Invited Speakers: Wharton Sinkler
This symposium will highlight the role of novel microscopy methods in the science and technology of catalysis and energy sources including hydrogen economy and fuel cells. The methods include in situ environmental microscopy for dynamic catalysis, Z-contrast/ tomography, ultra high vacuum methods, holography, and innovative sample stages. The topics include imaging, surface processes, nanomaterial architectures, spectroscopy, diffraction, electronic structure, data acquisition and combinatorial methods in understanding and developing materials and processes in catalysis. The symposium will feature invited talks by renowned international speakers and contributed papers and posters.
P05 Unraveling Magnetic Structure at the Nanoscale to Understand Magnetic Properties
Organizers: Marc De Graef, Yimei Zhu
Featured Invited Speakers: Stephen McVitie, Matthias Bode
Magnetic nanomaterials are among the most important classes of materials in today's rapidly advancing technology and telecommunication era. Their fascinating properties and applications are exclusively determined by their underlying magnetic structures. Quantitative characterization of the magnetic structure at the nanoscale is not a trivial undertaking and there is an urgent need to address the availability, advantages, and drawbacks of various techniques to solve material problems. This symposium will focus on magnetic structure characterization to reveal the underlying magnetic properties. Techniques will include, but are not limited to, Lorentz microscopy, magnetic force microscopy, off-axis electron-holography and other phase-retrieval methods, spin-polarized tunneling microscopy, spin-polarized scanning electron microscopy and polarized light microscopy. Structure-property relationships at both micro- and nano-scale will be discussed. Material classes include hard and soft magnets, thin film magnets, ferromagnetic shape memory alloys, magnetic semiconductors, materials with patterned magnetic structure or GMR / CMR effects, and modeling of magnetic micro- and nanostructures.
P06 Microscopy and Microanalysis of Nanotechnology
Organizers: David C. Bell, Douglas A. Blom
Featured Invited Speakers: Rich Superfine, Ned Thomas
Nanotechnology brings us the promise of devices and materials with extraordinary abilities; the first nanostructured materials and functional nano-devices have already been fabricated and can be classed as the first generation of nanotechnology. Microscopy and microanalysis of these devices for the purposes of product development and quality control present multiple issues. Nanotechnology utilizes the fundamental behavior of materials at the atomic and molecular scale. Imaging nano-devices is problematic due to the characteristics and the diverse materials used in fabrication. Traditional analytical procedures must be modified to encompass the diverse range of materials present and scaled to the minute quantities. Microscopy and microanalysis are critical for the development and commercialization of nanotechnology. In fact, the future of the entire nanotechnology revolution depends on them. This symposium presents a forum for discussion on state-of-the-art methods to characterize nanotechnology, both for research and commercial development of improved devices and production techniques. Open to all areas of nanotechnology research including but not limited to; nano-electronics, nano-photonics, micromechanical devices (MEMS) and Bio- MEMs. We seek examples that illustrate the adaptation of existing analytical and imaging techniques as well as those that present novel methods.
P07 The Characterization and Performance of Advanced Coatings and Thin Films
Organizers: Thad Adams, Paul Korinko.
Featured Invited Speakers: Phil Rack, Mark Weaver
This symposium will address the scientific issues related to phenomena in synthesis, characterization, and application of coatings and thin films. The objective of the symposium is to provide a multidisciplinary discussion at both the fundamental and applied levels of phenomena through which materials performance may be enhanced by advanced coating and thin film technologies. Specifi c topics include, but are not limited to: PVD and CVD processes, synthesis of nanostructures and nanoparticles, surface modification by plasma, ion and laser beam techniques, direct fabricated materials, thermal barrier coatings, biomedical coatings, high-temperature- oxidation- and corrosion-resistant coatings, thin films for electronic, optical and magnetic applications, coatings for space flight applications, coatings used in the automobile and environmental industries, corrosion resistant coatings, modeling, mechanical and tribological properties, interface properties and adhesion, advanced surface evaluation techniques, advanced optical and electron microscopy techniques, and ultrahard coating applications and testing.
P08 Metallography of High- Temperature Materials and Life Assessment
Organizers: George F. Vander Voort, Elena P. Manilova
Featured Invited Speakers: Alexander I. Rybnikov, Arun Gohkale
This symposium focuses on the use of metallographic techniques of all types to study high temperature materials: Fe-, Ni-, Co- or Ti-based alloys, ceramics and intermetallics. Topics include, but are not limited to, the use of microscopy in processing, alloy design and development, physical metallurgy, applications, service performance, remnant life assessment, and failure analysis. The methods include field metallography using replication techniques or portable microscopes, as well as traditional light optical, x-ray and electron metallographic laboratory methods. Particular interest is placed on studies of creep damage and estimation of the remaining component life.
Advances in Instrumentation and Techniques Symposia
A01 Microscopy and Microanalysis in the Real World: Textiles, Pharmaceuticals, Photonics, and Biomedical/ Forensics
Organizers: Janet Woodward, Danny Akin, Bev Maleeff, Barbara Hartman
Featured Invited Speakers: M. Sullivan, C. Humphrey, S. Zaki
Microscopy and microanalysis techniques are used in many "real world" settings in both the physical and life sciences. These real world applications target practical problem solving and can involve diagnostics, failure analysis, particle analysis, etc. Success often depends upon utilizing currently available techniques that can be applied rapidly with a high degree of reliability. Most often, the "real world" analysts operate under signifi- cant time and cost constraints. This year's symposium will be presented in a series of four "mini-symposia" on the topics: Textiles, Pharmaceuticals, Photonics, and Biomedical/ Forensics. Invited speakers will offer presentations that describe successful strategies for applying existing microscopic techniques to real world problem solving in these areas. In addition, contributed papers for platform or poster presentations on these and other "real world" topics are encouraged and welcomed. Textiles: This symposium will solicit papers in broad areas of microscopy and micro-spectroscopy concerning investigations of natural and synthetic fibers. Papers are sought on older and newer types of fibers, particularly as related to characteristics that modify properties in both woven and nonwoven applications. Reports from investigations on structure and chemical components of a wide scope are encouraged. Pharmaceuticals: The Pharmaceuticals mini-symposium will address the application of microscopy and microanalytic technologies with respect to specific areas of interest such as particle size analysis, product contamination and cell pathology, among others. In addition, an informal forum will be provided for discussion of thoughts and strategies related to regulatory and other issues faced in our laboratories. Photonics: Optical technologies are in every aspect of our daily lives - from the new organic LEDs for cell phones and cameras to surgical laser optics. This symposium will address the state of the art microscopic and microanalysis techniques utilized in this growing industry. Biomedical/Forensics: Welcome to the 21st century - where medical implants are routine surgeries, where the threat of bioterrorism hovers, and where we are entertained by various TV shows on forensic science. This symposium will provide a look into the "behind the scenes" real world investigations in this area of biomedical/ forensics.
A02 Advances in Visualizing Tissue Chemistry at the Cellular Level Using Infrared and Raman Microspectroscopy and Imaging
Organizers: Mike Jackson, Neil Lewis, Janie DuBois
Featured Invited Speakers: Gerwin Puppels, Richard Mendelsohn
Optical microscopy relies on specific chemical stains (H&E, fluorescently labelled antibodies, etc.) to provide sufficient contrast to explore tissue and cellular architecture and to allow assessment of tissue chemistry. A more direct assessment of tissue and cellular architecture and chemistry is provided by spectroscopic techniques that explicitly probe tissue properties. Infrared and Raman spectroscopy are among the most powerful of such techniques and provide information on isolated biological materials, single cells, biological fluids or complex tissues. This information may pertain to nuclear material, membrane dynamics or connective tissue components to give only a few examples. Importantly, an inherent advantage of such spectroscopic techniques is that information on multiple tissue/cell components is obtained in a single measurement. Furthermore, using advanced microscopic and imaging instrumentation spatial variations in tissue/cellular composition may also be assessed, essentially allowing tissue chemistry to be probed and visualised at the cellular level. This symposium will highlight recent advances in IR/Raman microscopic and imaging instrumentation. Particular emphasis will be placed on how these advances allow visualisation of the cellular processes underlying disease processes including malignant transformation, infection and neurological disorders.
A03 Quantitative Techniques in Biological Imaging
Organizers: Jay Jerome, Dave Piston
Featured Invited Speakers: Mike Marko, Rob Gourdie
Quantifying structure can signifi cantly enhance many types of experiments using imaging. This is particularly true for studies of changes in features at different times or with different treatments or in experiments correlating image data with information from other modalities such as biochemistry or physiology. The methods for quantifying image information are varied and run the gamut from very simple techniques to complex algorithms. The parameters that can be measured also vary from simple linear assessment to complex analysis of molecular concentration. This session will present a variety of methods available for biological image measurement and is open to anyone developing methods for quantifying structure as well as those applying quantitative methods to strengthen experimental design.
A04 Digital Imaging
Organizer: John Mackenzie
Featured Invited Speakers: P. C. Cheng
Digital imaging is replacing more and more of the imaging that we historically recorded on film. This symposium will focus on trying to better understand this digital technology and how best to apply it. We will exam archival issues as they relate to printers and CDs. We will explore how to best apply Photoshop for scientific digital imaging. We wish to emphasize the latest developments and directions in digital imaging technology. Finally, we invite papers that examine the question of how best to incorporate a digital workflow into the operation of a microscopy laboratory.
A05 Advances in Confocal Optical Microscopy Technology
Organizer: Steve Paddock
Featured Invited Speakers: Mary Dickinson, Wenbiao Gan
The symposium will include applications of the confocal optical microscope in both the biological and the physical sciences, and will also include advancements in associated technology for collecting optical sections. Emphasis will be placed on multi-dimensional imaging, including methods of imaging multiple-labeled specimens and issues posed by the analysis of multiple images and their display.
A06 Analysis of Protein Dynamics in Living Cells by Quantitative Light Microscopy
Organizer: Clare Waterman-Storer
Featured Invited Speakers: Derek Toomer, Klaus Hahn
Genetics, biochemistry, and molecular biology techniques have given rise to a vast encyclopedia of protein interactions mediating specific cell functions. However, in order to understand how protein machinery conspires to animate dynamic cell function, we need to know the "where, when, how much, and for how long" of protein behavior in living cells. This session will cover recent state-of-theart techniques in quantitative light microscopy as applied to analyzing the spatiotemporal control of molecular mechanisms underlying basic cellular processes. This includes analysis of nuclear processes such as transcription, signaling proteins that control various cell functions, cytoskeletal dynamics in regulating cell shape and motility, cell division, and membrane trafficking. The focus of the session will be to explain the how cutting edge technology was developed to answer important questions in cell biology.
A07 Biological Specimen Preparation and Labeling
Organizer: Ralph Albrecht
Featured Invited Speaker: Marek Malecki, Reiner Bleher
This symposium will emphasize emerging preparative and labeling technology for use in high resolution and correlative studies. It is becoming increasingly important to visualize, identify, and localize/co-localize multiple molecules and molecular complexes within cells and tissues. Currently our knowledge of the various molecular species within cells is expanding rapidly; determining the organization and movement of these components at the cellular and molecular level is a critical step in understanding cell function. Preparative and labeling methodology which permits the sequential examination of specimens in several microscopic modes is key to the success of these efforts.. Live specimens stained or labeled for examination via various modes of photon based imaging must be prepared for subsequent examination at substantially higher levels of spatial resolution using electron based or force based imaging (SEM, TEM, AFM). This requires preparative technology that maintains molecular and sub-molecular structure in the face of low and high accelerating voltages. Similarly, labeling methodology that is compatible with, and provides information from, more than one microscopic mode and generally at different levels of spatial resolution is also a requirement. Advances in preparative and labeling technology which facilitates correlative LM, TEM, and SEM will be addressed. Contributed platform presentations and posters dealing with novel preparative and labeling approaches are encouraged.
A08 Tomographic Techniques in Biological and Physical Sciences
Organizers: Michael Marko, John Henry Scott
Featured Invited Speakers: Wolfgang Baumeister, Paul Midgley
Electron tomography is now a mature technique, and is currently experiencing a period of rapid growth as emphasis shifts from technique development to application. Originally developed by the biological community, the technique has recently been adopted by materials scientists. Concurrently, other forms of tomography such as FIB tomography, atom-probe tomography, and x-ray microtomography are being developed to solve increasingly complex 3-dimensional characterization challenges. Imaging modes used for electron tomography now include not only conventional TEM and cryo-TEM, but also EFTEM, STEM and Z-contrast imaging, with some modes providing 3-D elemental maps. FIB and atom probe tomography are similar in that the specimen is ablated layer-by-layer, and the 3-D reconstruction is created by serial depth sectioning. However, the resolution and range of applications are quite different. X-ray microtomography offers the advantage of large depth of field, and does not require the specimen to be in a vacuum. Invited speakers will represent the state of the art in the development and application of several of these tomographic techniques. Contributed papers are sought in these areas as well as other micro-tomography techniques not mentioned above.
A09 FIB/Dual Platform Applications and Techniques in Biological and Physical Sciences
Organizers: Lucille Giannuzzi, Warren Moberly Chan
Featured Invited Speakers: Boris Mizaikoff, Hamish Frasier
Focused ion beam and combined FIB/SEM instrumentation is gaining popularity in microscopy laboratories. The unique imaging and milling capability of focused ion beams make it ideal for site-specific specimen preparation for multiple analytical instruments. The ability to introduce specific gases for deposition of various materials and sample modification has provided new and interesting applications for micro- and nanoprocessing. In addition, the incorporation of an SEM and FIB on the same platform may be used for 3D microstructural, elemental, and crystallographic analyses. Advances in computer automation of techniques also allows for unique applications and unattended microstructural analyses. This symposium encourages abstracts that discuss the development of new FIB instrumentation, applications, and techniques in both the physical and the biological sciences.
A10 Scanned Probe Microscopy: Probing Surfaces at the Nanoscale
Organizers: Phil Russell, Lou Germinario
Featured Invited Speakers: Larry Bottomley, Clayton Teague
The revolution in nanomaterials and nanotechnology research has both benefited from and led to the development of tools (sometimes referred to as microscopes) for their visualization, characterization and manipulation at the nanoscale. Leading among these are the scanned probe microscopes (known also as proximal probes), which can be used for interrogating and manipulating the structural, mechanical and chemical makeup of nanomaterials. The data thus obtained is often rendered in the form of topographic relief images (our familiar micrographs), which can be interpreted in a straightforward, intuitive way. Advances in applications of SPM to a wide variety of disciplines including, but not limited to, biology, nanofabrication, semiconductor and polymer science will be covered.
A11 In-Situ Characterization of Dynamic Processes by Variable Pressure Electron Microscopy
Organizers: Milos Toth, Eva Olsson
Featured Invited Speakers: Roger M. Leblanc, Harald Dobberstein
This symposium will solicit papers on variable pressure electron beam characterization of dynamic processes, charge-related contrast mechanisms and advances in environmental scanning and transmission electron microscopy theory, instrumentation, and applications. The unique role of environmental microscopy in electron beam characterization of dynamic processes stems from its ability to probe vacuum-intolerant and insulating materials. Its capacity to image surfaces and internal structures with high spatial resolution provides access to information that would otherwise be beyond reach and is of particular importance for nanoscience. The thermodynamic and electrostatic stabilization provided by an environmental gas facilitates in-situ investigation of a wide range of phenomena such as phase transitions induced by heating, cooling or changes in gas pressure, hydration-dehydration processes, behavior of films at gas-liquid interfaces, gas-surface reactions, beam-induced modification of insulating surfaces in low vacuum environments, and mechanical processes such as microcrack formation, buckling and fracture propagation. The dynamic nature of the physical mechanisms behind charge-stabilization of insulators gives rise to novel electron image contrast mechanisms, facilitating stable imaging of ferroelectric domains, defect distributions and spatial variations in electronic structure. The symposium will feature invited speakers illustrating state-of-the-art in-situ dynamic experiments.
A12 Micro X-ray Techniques: New Compositional and Structural Characterization Tools
Organizers: Harald Ade, Masashi Watanabe,
Featured Invited Speakers: Janos Kirz, Nestor Zaluzec
X-rays are used both as probes and as signals in a wide variety of microscopies, ranging from synchrotron radiation based Near Edge X-ray Absorption Fine Structure (NEXAFS) microscopy to Energy Dispersive X-ray analysis (EDX) in electron microscopes. This symposium will focus on the latest technological developments that are using x-rays as a tool for compositional and structural analysis and delineate the frontiers these tools are opening up.
A13 Accessorizing the SEM
Organizers: David Joy, John Henry Scott,
Featured Invited Speakers: Tom Prusnick, Del Redfern
The scanning electron microscope (SEM) is the most widely used electron- beam instrument because it is the best tool available. The large specimen chamber and the variety of imaging and analytical modes available provide unrivalled flexibility, as well as the inherent suitability for correlative microscopies. This symposium will survey the expanding imaging and analytical capabilities that result from adding new hardware and software to the SEM. Among the modes and technologies to be considered are: micro-Raman spectroscopy, energy filtering and multi-mode electron detectors; advanced X-ray spectrometers; X-ray fluorescence; X-ray microscopy; advanced electron backscatter diffraction (EBSD) and orientation imaging microscopy (OIM); and scanned probe microscopy (SPM) and nanoindentors in the SEM.
A14 Quantitative X-ray Microanalysis: Focus on Extraterrestrial and Terrestrial Specimens
Organizers: Paul Carpenter, Raynald Gauvin, Greg Meeker
Featured Invited Speakers: John Armstrong, Brendan Griffin, Mathias Procop
This symposium welcomes contributions from researchers active in any area of the development and application of quantitative X-ray microanalysis. Research on fundamental parameters measurement, correction procedure algorithms and software, Monte Carlo methods, spectrum generation, processing and modeling, compositional mapping, and precision and accuracy issues relating to microanalysis problems will be covered. Also discussed will be the challenges presented by analysis of insulating materials, rough surfaces, low energy x-rays, and variable pressure environments. Among applications of quantitative X-ray microanalysis, this symposium will have a focus on lunar, meteorite, and mineral specimens. Contributions are particularly encouraged in this area.
A15 Electron Energy-Loss and X-ray Absorption Spectroscopies: Focus on Anisotropic Properties
Organizers: Yasuo Ito, John W. Freeland
Featured Invited Speakers: Jun Yuan, Sarnjeet Dhesi
This symposium seeks to provide a forum for characterizing materials properties by EELS and XAS techniques that have complementary strengths while sharing a common theoretical background. The signifi cant advantage of EELS is its high spatial resolution, approaching the atomic-scale, whereas XAS has the advantages of high spectral resolution and polarization of the incident probe. The symposium will cover the theory, methods, and applications of these complementary techniques. A particular focus of the symposium will be the characterization of anisotropic properties of bulk materials and thin films. Anisotropy in crystal structure and charge density can manifest itself in corresponding magnetic, electrical, optical, and transport properties, with significance for applications such as switches, memory devices, and ionic conductors. These anisotropies can be characterized via the near-edge fine structure of ionization edges in EELS and with the X-ray nanoprobe, where polarization of the incident beam provides enhanced sensitivity to anisotropic properties. The symposium is expected to provide an active forum for interaction between the XAS and EELS communities. Contributions on all aspects of these techniques, including methods for improving spatial and spectral resolution, are welcome.
A16 Order in Disorder: Probing the Structure of Amorphous Materials
Symposium Organizers: John Mansfield, Paul Voyles
Featured Invited Speakers: Murray Gibson, Dave McKenzie
Many people make a sharp distinction between the structure of crystalline and amorphous materials. It has become clear, however, that nature is more complicated than that: amorphous materials can have varying degrees of order, and some materials have more than one distinct amorphous phase. Measuring nanoscale structure of amorphous materials has been notoriously difficult, but recent advances in instrumentation and computation have lead to new methods and discoveries. Among these is the fluctuation electron microscopy technique, which was first used by Treacy and Gibson to study amorphous semiconductors, and is now being applied to a wide range of materials. This symposium will discuss recent advances and applications of fluctuation microscopy, as well as other microscopy, spectroscopy, and diffraction techniques for measuring nanoscale order in amorphous materials.
A17 Metallurgical Failure Analysis: Present and Future
Organizer: Mike Stevenson
Featured Invited Speakers: Roch Shipley, Mike Stevenson
For decades now, Metallurgical Failure Analysis has served as a useful tool that engineers often employ to solve critical problems. First and foremost, metallurgical failure analysis aids in the understanding of how materials properties and processing will influence service performance. Metallurgical failure analysis is often employed as a reverse design methodology in which design refinements are identified through analysis of field or test failures. Another key area where metallurgical failure analysis is employed is in the reconstruction of incidents, where material failure processes can pinpoint or aid in determining the root cause of larger system failures. This symposium will highlight the current state of the art in Metallurgical Failure Analysis. specific topics to be highlighted will be: current and emerging analytical techniques, the incorporation of computational mechanical in failure analysis, metallography and failure analysis, electron microscopy and failure analysis, and root cause determination in metallurgical investigations.
A18 Metallography: Preparation, Application and Evaluation for the 21st Century
Organizers: George Blann, Arlan Benscoter
Featured Invited Speakers: George Vander Voort, Arlan Benscoter
When studying the microstructural characteristics of metals and materials, metallographic specimen preparation has been, and continues to be the fundamental method used. Preparation methods have been revolutionized over the past decade and in the early years of this new millennium, new cost effective and repeatable methods are being used which produce excellent results enabling the materials scientist to accurately evaluate a wide range of material types. Improvements in preparation and optical equipment interfaced with computer systems, produces state-of-the-art results enabling the materials industry to develop universal standards of preparation and evaluation. This session will acquaint individuals with the progress made in preparation, application and evaluation of materials being used now and into the 21st century.
A19 Stereology and 3D Digital Imaging
Organizers: Bob DeHoff, Jim Steele
Featured Invited Speakers: Rudolf Hilfer, Peter Voorhees
The term "stereology" is used to describe the set of methods that allow the extraction of quantitative 3D structural information from observations of 2D sections. In modern stereological practice, no assumptions are made about the structure (so-called "design-based stereology") and the geometric measuring probes are randomized. Recent work has focused on the use of 3D digital images to represent 3D microstructures, and to provide estimates of connectivity in porous media. The general stereological problem of obtaining unbiased estimates of 3D microstructural parameters, such as volume fraction (VV), surface density (SV), lineal feature density (LV), number density (NV), and 3D connectivity, will be considered in this symposium. specific issues to be addressed are the 3D structural information that can be obtained from: 2D section probes; the "physical dissector," "optical dissector," and "optical fractionator" methods; n-point correlation functions; computer-assisted microtomography methods; and 3D digital reconstructions. Contributions on applications of sterelogy in biological and materials sciences are welcomed.
Technologists' Forum
Organizer: Cathy Johnson
X31 Platform Session: Advanced Techniques
Featured Invited Speakers: John J. Friel, Simon Watkins
Technology is ever changing, so too is the methodology required to obtain success in today's working laboratory. This year's symposium will feature presentations on methodologies that are not commonly used by the typical microscopy laboratory, but which are rapidly gaining recognition for providing unique information. Whether performed on-site or accessed by outsourcing to a contract lab facility, today's technologist must keep abreast of new analytical trends to remain informed and better prepared for the future. Example methodologies include: high-pressure freeze substitution, Raman microscopy, live cell imaging, scanning X-ray microscopy, quantum dot immunolabeling, and spectral imaging.
X32 Special Topics: Digital Cameras & Microscopy
Digital imaging has become an integral part of today's laboratory. Session presentations will cover the use and optimization of digital cameras in conjunction with light microscopy. The latest in camera hardware/ software will be presented. Speed, resolution, the merits of detector cooling, recording dynamic processes and stage/focus automation are just some of the features that will be addressed.
X33 Roundtable Discussion: LIMS - Laboratory Information Management Systems
Specimen tracking and information management is a critical operation for all laboratory facilities. Panel members will discuss how best to document and track specimens through the laboratory environment. Topics for discussion include: commercial and in-house-designed systems, considerations when establishing the tracking database, chain of custody concerns, data security, regulatory compliance, customer data access, and data archiving.
Topics for Contributed Sessions


Biological Sciences

C01 Biological Sciences - General B01-B07
C02 Biological Microanalysis
C03 Biological Specimen Preparation A07
C04 Biomaterials B02
C05 Biomedical Applications A02
C06 Biomimetics
C07 Blood / Immunology
C08 Botany B03
C09 Cell Biology B07
C10 Cytochemistry (Histochemistry, Immunochemistry,In-Situ Hybridization)
C11 Cytoskeleton B07
C12 Developmental / Reproductive Biology
C13 Entomology
C14 Histology X43
C15 Live Cell Imaging B04, A06,X15
C16 Macromolecules B05
C17 Microbiology B06
C18 Microorganisms B03
C19 Molecular Biology
C20 Neurobiology B04
C21 Parasitology
C22 Pathology
C23 Structural Biology B07, A03
C24 Ultrastructure (Cells, Tissues, Organ Systems)
C25 Vascular Corrosion Casting B01

Physical Sciences

C26 Physical Sciences - General P01-P08
C27 Amorphous Materials A16
C28 Catalysts P04
C29 Ceramics
C30 Composites
C31 Ferroelectrics
C32 Films / Coatings P07
C33 Geology / Mineralogy
C34 Interfaces P01
C35 Magnetic and Superconducting Materials P05
C36 Metals and Alloys P08
C37 Modulated Structures
C38 Nanostructured materials P02
C39 Nanotechnology P06
C40 Oxidation / Corrosion
C41 Particle Analysis
C42 Pharmaceuticals P03, A01
C43 Phase Transformations P02
C44 Polymers
C45 Porous Materials
C46 Radiation Effects in Materials
C47 Self-Assembly P03
C48 Semiconductors
C49 Specimen Preparation for Materials Sciences
C50 Surfaces

Advances in Instrumentation and Techniques

C51 Advances in Instrumentation and Technique - General A01-A19
C52 Instrumentation Performance & Development
C53 Electron Optics and Aberration Correction X01
C54 Transmission Electron Microscopy (TEM)
C55 Scanning Transmission Electron Microscopy (STEM) X53
C56 Electron Holography X54
C57 High-Resolution Electron Microscopy (HREM)
C58 Analytical Electron Microscopy (AEM)
C59 Electron Energy-Loss Spectroscopy (EELS) / Energy-Filtered TEM (EFTEM) A15
C60 Convergent Beam Electron Diffraction (CBED) X52
C61 In-situ TEM A11
C62 Scanning Electron Microscopy (SEM) A13
C63 Low-voltage SEM
C64 Variable Pressure SEM (VPSEM/ESEM) A11, X18
C65 Electron Backscatter Diffraction (EBSD) A13
C66 X-ray Spectrometry A13
C67 Quantitative X-ray Microanalysis A14, X51
C68 Spectral Imaging
C69 X-ray Imaging, Diffraction and Spectroscopy A12, A15
C70 Crystallography
C71 Tomographic Methods B05, A08, A19, X61
C72 Focused Ion Beam (FIB) A09
C73 Surface Analysis techniques
C74 Atom Probe Field Ion Microscopy (APFIM)
C75 Scanned Probe Microscopy (SPM) A10
C76 Metallography and Metallographic Specimen Preparation P08, A18,X11
C77 Stereology A19, X43
C78 Optical (Light) Microscopy A06
C79 Confocal Microscopy A05, X14,X42
C80 Multi Photon Excitation Microscopy X42
C81 Optical Fluorescence Microscopy X15, X42
C82 Infrared and Raman Microscopy and MicroanalysisA02
C83 Molecular Spectroscopy X42
C84 Correlative Microscopy
C85 Combinatorial Methods
C86 Cryogenic Techniques and Methods B05, P03
C87 In-vivo Imaging
C88 Digital Image Acquisition, Processing, and Analysis A04, X16,X17
C89 Computational Methods
C90 Remote Microscopy and Collaboration
C91 Education in Microscopy and Microanalysis
C92 Failure Analysis A17, X13
C93 Forensic Science A01
C94 Industrial "Real World" Microscopy A01
C95 Quality Systems and Standards
C96 Technologists' Forum X31-X33
C97 Core Facility Management
C98 User Facilities
C99 Corporate Session X72
Tutorials
X41 Introduction to Fluorescence and Image Correlation Spectroscopy
Instructor: Paul Wiseman
This tutorial will be an introduction to fluorescence correlation spectroscopy (FCS) with a particular focus on its imaging analog called image correlation spectroscopy (ICS). An overview of the history and theory of these fluctuation microscopy methods will be presented along with a description of the microscopy instrumentation required to perform such measurements. The second half of the tutorial will describe biological applications of image correlation techniques in both cell biology and neuroscience. We will discuss specific applications such as measuring protein transport (both flow and diffusion), receptor clustering and interactions for proteins involved in the adhesion machinery of adherent and migratory cells. The tutorial will also discuss applications in neuroscience for measurement of dendritic spine densities in hippocampus tissue slices.
X42 Molecular Tags: A Multi- Color, Multi-Scale Approach to Recombinant Protein Analysis
Instructor: Guido Gaietta
Since their first introduction in the early '80s, fluorescent proteins have proven incredibly valuable to the study of many proteins directly in their native environment. Recent advancements in molecular tagging have given us the ability to follow a target protein at different resolution levels, to examine its interactions with other proteins and ultimately, to study its function. This tutorial will provide a general overview of two tagging systems: GFP (and its variants) and 4Cys/FlAsH. We will examine the basics of each system, compare them with one another and explore their applications to confocal, 2-photon and electron microscopy. Examples of FRET, FRAP and FALI will also be covered during the presentation.
X43 Implementing Design- Based Stereology
Instructor: Daniel A. Peterson
Quantitative histology has become an increasingly important form of analysis in biomedical research. Some form of outcome measurement, frequently cell number, is required to provide data for statistical analysis to accept or reject the hypothesis. For microscopic analysis, this requires counting objects (cells) that are embedded within a larger structure (tissue). However, these cells must be counted from sections produced from the tissue. As a result of sectioning the tissue and visualizing the cells through an objective lens that produces a depth of field, one must consider potential artifacts that could introduce error into the determination of the number of objects. These sources of error can be addressed through the use of systematic three-dimensional sampling probes collectively known as design-based stereology. This tutorial will review the principles involved and illustrate practical examples of implementing design-based stereology.
X51 Energy Dispersive X-ray Spectrometry in the Scanning Electron Microscope
Instructor: Dale E. Newbury
Energy dispersive x-ray spectrometry (EDS) performed in the scanning electron microscope (SEM) has become a basic procedure supporting a vast range of applications. Most often applied to practical problems as a qualitative analysis technique, SEM/ EDS is actually capable of achieving quantitative results with a small error budget, +5% relative or better. The steps to achieve this level of accuracy with SEM/EDS will be discussed: (1) careful measurement science practices for electron-excited x-ray spectrometry; (2) robust qualitative analysis at all levels: major (C > 0.1 mass fraction), minor (0.01 < C < 0.1), and trace (C < 0.01); (3) statisticallystable peak and background deconvolution; and (4) quantitative matrix corrections.
X52 Electron Diffraction for Modern Materials Characterization
Instructor: Jian-Min Zuo
Electron diffraction patterns are routinely recorded in electron microscopes to obtain quantitative structure information about crystal phases and orientation relationships. The recent developments of field-emission gun electron microscopes, energy-filters, digital detectors and computer simulations have significantly improved both the sensitivity and quantification of electron diffraction. Meanwhile, new materials development focuses increasingly on new structures at nanoscale, where the spatial resolution of electron diffraction is an advantage. This tutorial starts with basic knowledge of electron diffraction and then covers its application for modern materials characterization. Topics include: diffraction modes in TEM, crystal diffraction and indexing, the convergent beam geometry and diffraction of nanomaterials. The level of the tutorial is aimed at TEM users who have practical knowledge of electron diffraction, but are interested in improving their skills on interpretation and learning new quantitative techniques of electron diffraction.
X53 Practical Scanning Transmission Electron Microscopy
Instructor: David. A. Muller
Modern commercial field emission transmission electron microscopes (TEM) have the potential to produce atomic-resolution scanning transmission electron microscopy (STEM) images if properly aligned and installed. This tutorial will cover practical and theoretical aspects necessary to optimize the microscope for STEM. Topics covered: interpreting Ronchigrams, choosing the right apertures and camera lengths, bright and dark field imaging, how to make your STEM images look like they were taken on a TEM, Z-contrast and strain mapping, sample thickness dependence of STEM images and imaging artifacts.
X54 Practical Electron Holography
Instructor: Molly McCartney
Electron holography is an evolving technique that is gaining increased interest in the microscopy community for characterization of electric fields and magnetic materials in addition to its original application for resolution enhancement. This tutorial will cover a wide range of aspects including the history of the technique and theoretical and experimental issues. In particular, the source and implications of the phase shifts measured in the reconstructed holograms will be discussed. Emphasis will be placed on practical aspects of electron holography including the experimental set-up, reconstruction and the precision and accuracy of the reconstructed phase images. Applications to semiconductor junction delineation, piezoelectric fields and magnetic thin films will be discussed.
X61 Techniques for Electron Tomography in Biological and Materials Science
Instructor: Mike Marko
There are a wide variety of applications for electron tomography, and a correspondingly wide variety of imaging modes and data collection strategies. In biological sciences, low-dose, low-temperature tomography of unstained frozen-hydrated specimens is of great interest, while tomography of stained, thick plastic sections at high accelerating voltage is essential for other studies. In materials science, techniques such as HAADF STEM tomography are needed for crystalline specimens. Elemental mapping using EFTEM or STEM is being applied in both fields. The tutorial will attempt to cover all known forms of transmission electron tomography, and describe how each is implemented for an appropriate application.
Additional Sessions
X71 Ask The Experts
Organizer: Lucille Giannuzzi
The Ask-the-Experts session takes on a different format this year to provide programming closer to the time of the annual Microscopy and Microanalysis (M&M) meeting, and to better address the needs of the meeting attendees. Rather than listing topics in the Call for Papers, which must be produced nearly a year before the meeting, an opportunity to submit questions to experts attending M&M 2004 will be provided at the time the program is posted on the meeting web site in the months leading up to the meeting. This link will allow participants to submit questions, and name the expert to whom the question is addressed, as they browse the M&M 2004 program on-line. The Ask-the- Experts organizers will then seek to schedule a time to address topics of significant interest during the meeting. The schedule of topics will be announced and posted to the meeting website a couple of weeks before the early registration deadline, so that potential M&M 2004 attendees can schedule in the topics of interest to them. Please plan to participate both in the topic selection and the sessions, so that we can gauge whether this change of format is beneficial!
X72 Corporate Session
Organizer: Jan Rignalda
This session will be aimed at new solutions from manufacturers or corporate sponsors that have scientific merit and offer unique capabilities for the scientific community. This is not a session for advertising the latest engineering widget, rather a forum to show how a new product or even a new application of an older product can be of significant interest to us. Since there is some issue with predicting the future, the format this year will be flexible to the extent that this may be a forum for "late breaking scientific developments." although this will depend on the interest shown ahead of time.
X73 Late-Breaking Poster Session
Organizer: Ian M. Anderson
The late-breaking poster session provides an avenue for presenting results not submitted before the February 16 paper submission deadline. Contributions for this session will be accepted until FRIDAY, JULY 16, 2004. Because of space limitations, we cannot guarantee acceptance of these contributions, but will attempt to accommodate as many as possible. Late-Breaking Poster submissions will NOT appear in the proceedings.



Nestor J. Zaluzec / zaluzec@microscopy.com